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BIOTAGE allele quantification software
Allele Quantification Software, supplied by BIOTAGE, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/allele+quantification+software/allele+quantification+software/pmc03162605-251-31-34
Average 90 stars, based on 1 article reviews
allele quantification software - by Bioz Stars, 2026-10
90/100 stars

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Related Articles

Software:

Article Title: Evaluation of a new pooling strategy based on leukocyte count for rapid quantification of allele frequencies.
Article Snippet: .. The areas under the curves were determined, and the respective allele frequencies (given as percentage) for the analyzed SNPs were calculated by the instrument’s allele quantification software (Biotage). ..

Article Title: Aryl hydrocarbon receptor nuclear translocator (ARNT) gene as a positional and functional candidate for type 2 diabetes and prediabetic intermediate traits: Mutation detection, case-control studies, and gene expression analysis
Article Snippet: .. Allelic specific quantitation of both cDNA and genomic DNA samples was determined using the same assay for pyrosequencing on a PSQ 96 Pyrosequencer (Biotage, Inc, Uppsala, Sweden), with peak height quantified using Allele Quantification software (Biotage, Inc). ..

Article Title: Impact of Abnormal DNA Methylation of Imprinted Loci on Human Spontaneous Abortion.
Article Snippet: Currently, there is a growing concern regarding the safety of assisted reproductive technology (ART) due to increased risk of spontaneous abortion (SA) and imprinting disorders in ART-conceived offspring.. Early investigations suggested that aberrant genetic imprinting may be related to pregnancy loss; however, few studies have used human tissue specimens.. Here the DNA methylation patterns of 3 imprinted genes, including maternally inherited GRB10 and the paternally inherited IGF2 and PEG3 genes, were evaluated in human chorionic villus samples by pyrosequencing and bisulfite sequencing polymerase chain reaction.

Article Title: Activating Transcription Factor 6 (ATF6) Sequence Polymorphisms in Type 2 Diabetes and Pre-Diabetic Traits
Article Snippet: Both cDNA and genomic DNA were typed using the same assay by pyrosequencing, using a PSQ-96 machine (Biotage) with a universal biotinylated primer. .. Peak heights for the two alleles were quantified in both cDNA and genomic DNA using Allele Quantification software (Biotage), which was designed for quantification of pooled genotypes and which provides a ratio (percentage of total peak summing to 100%) for each allele. ..

Article Title: The Effect of ACACB cis -Variants on Gene Expression and Metabolic Traits
Article Snippet: .. Allelic specific quantitation of both cDNA and genomic DNA samples was determined using the same assay for pyrosequencing on a PSQ 96 Pyrosequencer (Biotage, Uppsala, Sweden) with peak height quantified using Allele Quantification software (Biotage, Uppsala, Sweden). ..

Article Title: Mandipropamid targets the cellulose synthase‐like PiCesA3 to inhibit cell wall biosynthesis in the oomycete plant pathogen, Phytophthora infestans
Article Snippet: The pyrosequencing primer Pyr_Pinf_Ces3seq (Table S1, see Supporting Information) was then added to the biotinylated strand and the mixture was sequenced using Pyromark ID (Biotage). .. The gene copy number was assessed using Biotage allele quantification software. ..

Quantitation Assay:

Article Title: Aryl hydrocarbon receptor nuclear translocator (ARNT) gene as a positional and functional candidate for type 2 diabetes and prediabetic intermediate traits: Mutation detection, case-control studies, and gene expression analysis
Article Snippet: .. Allelic specific quantitation of both cDNA and genomic DNA samples was determined using the same assay for pyrosequencing on a PSQ 96 Pyrosequencer (Biotage, Inc, Uppsala, Sweden), with peak height quantified using Allele Quantification software (Biotage, Inc). ..

Article Title: The Effect of ACACB cis -Variants on Gene Expression and Metabolic Traits
Article Snippet: .. Allelic specific quantitation of both cDNA and genomic DNA samples was determined using the same assay for pyrosequencing on a PSQ 96 Pyrosequencer (Biotage, Uppsala, Sweden) with peak height quantified using Allele Quantification software (Biotage, Uppsala, Sweden). ..

Methylation:

Article Title: Impact of Abnormal DNA Methylation of Imprinted Loci on Human Spontaneous Abortion.
Article Snippet: Currently, there is a growing concern regarding the safety of assisted reproductive technology (ART) due to increased risk of spontaneous abortion (SA) and imprinting disorders in ART-conceived offspring.. Early investigations suggested that aberrant genetic imprinting may be related to pregnancy loss; however, few studies have used human tissue specimens.. Here the DNA methylation patterns of 3 imprinted genes, including maternally inherited GRB10 and the paternally inherited IGF2 and PEG3 genes, were evaluated in human chorionic villus samples by pyrosequencing and bisulfite sequencing polymerase chain reaction.



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Representation of the two sequence targets in the exon III of the OXTR gene. Legend: Representation of OXTR gene with the CpG island in green and the location of exon III in blue . The dashed lines show the amplification of the region analyzed that contains two targets, OXTR1 OXTR2. The target sequences are in the exon III of OXTR gene ( OXTR1 —cat. PM00016821 and OXTR2 —cat. PM00016828, QIAGEN), using the <t>PyroMark</t> PCR Kit (QIAGEN). Both regions are in the CpG island the comprise exons I–III. The OXTR1 target sequence has 34 pb with 4 CpG sites analyzed and the OXTR2 target sequence has 34 bp with 5 CpG sites analyzed
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Representation of the two sequence targets in the exon III of the OXTR gene. Legend: Representation of OXTR gene with the CpG island in green and the location of exon III in blue . The dashed lines show the amplification of the region analyzed that contains two targets, OXTR1 OXTR2. The target sequences are in the exon III of OXTR gene ( OXTR1 —cat. PM00016821 and OXTR2 —cat. PM00016828, QIAGEN), using the <t>PyroMark</t> PCR Kit (QIAGEN). Both regions are in the CpG island the comprise exons I–III. The OXTR1 target sequence has 34 pb with 4 CpG sites analyzed and the OXTR2 target sequence has 34 bp with 5 CpG sites analyzed
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Representation of the two sequence targets in the exon III of the OXTR gene. Legend: Representation of OXTR gene with the CpG island in green and the location of exon III in blue . The dashed lines show the amplification of the region analyzed that contains two targets, OXTR1 OXTR2. The target sequences are in the exon III of OXTR gene ( OXTR1 —cat. PM00016821 and OXTR2 —cat. PM00016828, QIAGEN), using the <t>PyroMark</t> PCR Kit (QIAGEN). Both regions are in the CpG island the comprise exons I–III. The OXTR1 target sequence has 34 pb with 4 CpG sites analyzed and the OXTR2 target sequence has 34 bp with 5 CpG sites analyzed
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Representation of the two sequence targets in the exon III of the OXTR gene. Legend: Representation of OXTR gene with the CpG island in green and the location of exon III in blue . The dashed lines show the amplification of the region analyzed that contains two targets, OXTR1 OXTR2. The target sequences are in the exon III of OXTR gene ( OXTR1 —cat. PM00016821 and OXTR2 —cat. PM00016828, QIAGEN), using the <t>PyroMark</t> PCR Kit (QIAGEN). Both regions are in the CpG island the comprise exons I–III. The OXTR1 target sequence has 34 pb with 4 CpG sites analyzed and the OXTR2 target sequence has 34 bp with 5 CpG sites analyzed
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BIOTAGE aq (allele quantification) software module
Representation of the two sequence targets in the exon III of the OXTR gene. Legend: Representation of OXTR gene with the CpG island in green and the location of exon III in blue . The dashed lines show the amplification of the region analyzed that contains two targets, OXTR1 OXTR2. The target sequences are in the exon III of OXTR gene ( OXTR1 —cat. PM00016821 and OXTR2 —cat. PM00016828, QIAGEN), using the <t>PyroMark</t> PCR Kit (QIAGEN). Both regions are in the CpG island the comprise exons I–III. The OXTR1 target sequence has 34 pb with 4 CpG sites analyzed and the OXTR2 target sequence has 34 bp with 5 CpG sites analyzed
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Average 90 stars, based on 1 article reviews
aq (allele quantification) software module - by Bioz Stars, 2026-10
90/100 stars
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Image Search Results


Representation of the two sequence targets in the exon III of the OXTR gene. Legend: Representation of OXTR gene with the CpG island in green and the location of exon III in blue . The dashed lines show the amplification of the region analyzed that contains two targets, OXTR1 OXTR2. The target sequences are in the exon III of OXTR gene ( OXTR1 —cat. PM00016821 and OXTR2 —cat. PM00016828, QIAGEN), using the PyroMark PCR Kit (QIAGEN). Both regions are in the CpG island the comprise exons I–III. The OXTR1 target sequence has 34 pb with 4 CpG sites analyzed and the OXTR2 target sequence has 34 bp with 5 CpG sites analyzed

Journal: BMC Neuroscience

Article Title: Epigenetic evidence for involvement of the oxytocin receptor gene in obsessive–compulsive disorder

doi: 10.1186/s12868-016-0313-4

Figure Lengend Snippet: Representation of the two sequence targets in the exon III of the OXTR gene. Legend: Representation of OXTR gene with the CpG island in green and the location of exon III in blue . The dashed lines show the amplification of the region analyzed that contains two targets, OXTR1 OXTR2. The target sequences are in the exon III of OXTR gene ( OXTR1 —cat. PM00016821 and OXTR2 —cat. PM00016828, QIAGEN), using the PyroMark PCR Kit (QIAGEN). Both regions are in the CpG island the comprise exons I–III. The OXTR1 target sequence has 34 pb with 4 CpG sites analyzed and the OXTR2 target sequence has 34 bp with 5 CpG sites analyzed

Article Snippet: We analyzed the data using PyroMark Q24 allele quantification software, version 2.0.6 (QIAGEN), which allowed us to calculate the proportional methylation.

Techniques: Sequencing, Amplification